Seminars of the Focus Area Complex Systems

Prof. Dr. C. Beta, Prof. Dr. K. Dethloff, Prof. Dr. R. Engbert, Prof. Dr. M. Holschneider, Prof. Dr. W. Huisinga, Prof. Dr. Ralf Metzler, Prof. Dr. A. Pikovsky, Prof. Dr. S. Reich, Prof. Dr. M. Rosenblum, Prof. Dr. G. Rüdiger, Prof. Dr. T. Scheffer, Prof. Dr. F. Scherbaum, Prof. Dr. J. Selbig, Prof. Dr. F. Spahn

Seminar

Speaker: Marco Tarantola, MPI for Dynamics and Self-Organization, Göttingen

Title: Mechanics and dynamics of cellular contacts: From Dictyostelium discoideum adhesion to mammalian cardiac fibrosis * Joint Event: Colloquium on Complex and Biological Systems / Seminar of the GRK 1558

Time: Fri, Nov 15, 2013, 10:15am

Place: bldg 28, room 0.108

Abstract:
Cell-cell as we as cell-substrate contacts are of uttermost importance in many biological processes. For the eukaryote Dictyostelium discoideum (D.d), cell-substrate adhesion still remains to be fully understood, as D.d lacks integrins. Therefore, it is ideally suited to study alternative adhesion properties reflective of eukaryotic evolution. In Single Cell Force Spectroscopy (SCFS), we attach single cells to a cantilever and probe quantitatively mechanics of cell-substrate adhesion. We find that substratum adhesion is dramatically reduced during the first 6 hrs of starvation-induced development. Furthermore, D. d. synchronizes itself during starvation inducing waves of cAMP. We have quantified these collective phenomena based on cell-cell and cell-substrate contacts by applying electric impedance measurements (ECIS) and showed that cells seeded on electrodes provoke impedance oscillations. The results provide a quantitative understanding of the overall cell morphology dynamics during early starvation. Another system ideally suited to study cellular contacts is mammalian cardiac fibrosis: here, our goal is to obtain passive electrical and mechanical properties of the multicellular substrate and thus quantitative results on the electromechanical coupling of primary co-cultures of rat cardiac myocytes (CMs) and fibroblasts (Fbs) in vitro: using impedance spectroscopy and atomic force microscopy, we are able to quantify adhesion kinetics, contractility and connectivity between CMs and Fbs. As CMs and Fbs represent the two major cell types of the heart and are held responsible for fibrotic arrhythmia, we hereby contribute to a deeper mechanistic insight into this cardiovascular disease.

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Past NLD Seminars (1994-2007) & (2008 ...)

Students' seminar: Theoretical Physics, PIK, Modeling & TSA Berlin-Potsdam-Colloquia: PhysGesellschaft Berlin, TU Berlin, Pro Physik, AIP, AEI, MPI-KGF, GFZ, HMI, PIK, AWI, Max Planck Institute for the History of Science, Mathematik, DPG Disputationen, & Vorschau UP

Udo Schwarz, Zentrum für Dynamik komplexer Systeme,
Universität Potsdam, Campus Golm Karl-Liebknecht-Str. 24, 14476 Potsdam, building 28, room 2.107
Phone: (+49-331) 977-1658, Fax : (+49-331) 977-1045

Email: Udo.Schwarz AT uni-potsdam.de

DFG SFB 1294

DFG Sonderforschungsbereich 1294 Data assimilation

DFG SPP 1488

DFG Schwerpunktprogramm 1488 Planetmag


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